Primetime for Protein Arrays: related news

Primetime for Protein Arrays

Protein microarrays allow researchers to analyze expression, protein interactions, and more. Recent advances eliminate those pesky purification steps, making these arrays more reliable and stable.

Deconstructing Green Fluorescent Protein

Green fluorescent protein (GFP) has been reassembled from two pieces, a large fragment 214 amino acids in length that is produced recombinantly (GFP 1−10) and a short synthetic peptide corresponding to the 11th stave of the β-barrel that is 16 amino acids long (synthetic GFP 11), following a system developed by Waldo and co-workers (Cabantous, S.; et al. Nat. Biotechnol. 2005, 23, 102−7) as an in vivo probe for protein association and folding. We demonstrate that the reassembled protein has identical absorption and excited-state proton transfer dynamics as a whole protein of the identical sequence. We show that the reassembled protein can be taken apart and the peptide replaced with a different synthetic peptide designed to perturb the chromophore absorption.

Protein folding: terahertz absorption spectroscopy detects changes in protein-water network

(Nanowerk News) Water is no passive spectator of biological processes; it is an active participant. Protein folding is thus a self-organized process in which the actions of the solvent play a key role. So far, the emphasis in studies of protein folding processes has been on observation of the protein backbone and its side chains.

Native Conformation at Specific Residues in Recombinant Inclusion Body Protein in Whole Cells Determined with Solid-State NMR Spectroscopy

Inclusion bodies are insoluble aggregates that are formed by bacteria to store excess recombinant protein produced during expression. The structure of the protein in inclusion bodies is poorly understood but it has been hypothesized that the protein may form misfolded β sheet aggregates. This paper presents an isotopic labeling and solid-state nuclear magnetic resonance approach to determine the secondary structure of individual residues within a recombinant influenza virus “FHA2� protein in inclusion bodies. The inclusion bodies were studied either in the context of the unlysed hydrated E. coli cells or in the hydrated pellet formed from centrifugation of the material insoluble in the cell lysate. The native structure of FHA2 is predominantly helical and native helical structure was also observed for several specific residues in the

Amino Acid Selective Cross-Saturation Method for Identification of Proximal Residue Pairs in a Protein'Protein Complex

Amino Acid Selective Cross-Saturation Method for Identification of Proximal Residue Pairs in a Protein−Protein Complex

Protein Science

This journal publishes full-length original research, peer reviews, and book reviews on all scientific aspects of protein molecules. Significant advancements relating to the chemical, biophysical, and recombinant determination of protein structure have been reported in Protein Science.

Penn Study Finds Way to Prevent Protein Clumping Characteristic of Parkinson's Disease

Researchers have identified a protein from a most unlikely source--baker's yeast--that might protect against Parkinson's disease. By introducing the yeast protein Hsp104 into animal models of Parkinson's disease, researchers prevented protein clumping that leads to nerve cell death, characteristic of the disorder. Full story

Study Finds Way to Prevent Protein Clumping Characteristic of Parkinson's Disease

Newswise - Researchers at the University of Pennsylvania School of Medicine have identified a protein from a most unlikely source -- baker's yeast -- that might protect against Parkinson's disease. More than a million Americans suffer from Parkinson's disease, and no treatments are available that fundamentally alter the course of the condition. By introducing the yeast protein Hsp104 into animal models of Parkinson's disease, researchers prevented protein clumping that leads to nerve cell death characteristic of the disorder.

Germans link allergies to gene and skin protein

Munich - German scientists Wednesday linked allergy illnesses including dermatitis, hay fever and asthma to a gene in 8 per cent of European populations that deprives them of an essential skin protein. A study of 3,000 Munich schoolchildren produced the proof that a lack of the protein filaggrin, found in skin cells, is associated with skin allergies. The study tracked down variants of the gene that produces the protein.

A Protein's Path to the "Chamber of Doom"

PROTEIN, PROTEASOME, 26S, MISFOLDED PROTEIN, ALZHEIMER'S, PARKINSON'S, ATPASES,NANOMACHINES, TECHNION, GLICKMAN, ISRAEL, UNIVERSITY OF TEXAS

Surprising Details Of Evolution Of Protein Translation Revealed

A new study of transfer RNA, a molecule that delivers amino acids to the protein-building machinery of the cell, challenges long-held ideas about the evolutionary history of protein synthesis.

Bio-Rad Describes Methods for Protein A Antibody Purification

Bio-Rad Laboratories, Inc. has announced the availability of a technical note (tech note 5712A) entitled, “The Profinia™ Protein Purification System Simplifies Antibody Purification with Protein A�.

Study reveals surprising details of the evolution of protein translation

A new study of transfer RNA, a molecule that delivers amino acids to the protein-building machinery of the cell, challenges long-held ideas about the evolutionary history of protein synthesis.

Study reveals surprising details of the evolution of protein translation

CHAMPAIGN – A new study of transfer RNA, a molecule that delivers amino acids to the protein-building machinery of the cell, challenges long-held ideas about the evolutionary history of protein synthesis.

Researchers Discover New DNA Binding Activity of E. coli Protein

Northeastern University scientists have discovered a new and unique DNA binding property of a protein in E. coli. Penny J. Beuning, Assistant Professor in the Department of Chemistry and Chemical Biology, spent the last two years researching double and single-stranded DNA binding of E. coli DNA polymerase III alpha protein and notes that her findings have potential for developing a new antibacterial target.

Bio-Rad Shows Method to Obtain Tag-Free, Native Protein in Less than an Hour

Bio-Rad Laboratories, Inc. has announced the availability of a new technical note (tech note 5652A) entitled “Profinity eXact™ Fusion-Tag System Performs On-Column Cleavage and Yields Pure Native Protein From Lysate in Less Than an Hour,� which compares methods used for fusion protein construction, tag cleavage involving the addition of protease, and subsequent steps involved for post-purification tag and protease removal, to the novel Profinity eXact system.

Genome Sequencer FLX System plus NimbleGen Sequence Capture Arrays Help Unravel the Cryptic Genetic Changes in Hematological Malignancies

PENZBERG, Germany--(BUSINESS WIRE)--Jul 8, 2008 - The Cancer Genomics Group at Barts and The London Medical School is researching leukemia with the aquisition of the state-of-the-art Genome Sequencer FLX System paired with the Roche NimbleGen Sequence Capture arrays. Researchers, led by Professor Brian Young, will use the combined technology of 454 Sequencing and NimbleGen Sequence Capture Arrays to uncover the genetic changes that are involved in the development of leukemia. The new sequence information will be linked to an existing extensive clinical and cytogenetic database to investigate the relationship between genetic change and the clinical features of this devastating disease. The researchers expect that the new data could have implications for the future therapy and clinical outcome of hematological malignancies.

Direct Observation of the Gating Step in Protein Electron Transfer: Electric-Field-Controlled Protein Dynamics

sica/INQUIMAE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. 2, piso 1, C1428EHA Buenos Aires, Argentina

X-ray Structure of Snow Flea Antifreeze Protein Determined by Racemic Crystallization of Synthetic Protein Enantiomers

Institute for Biophysical Dynamics, Department of Chemistry, and Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, and Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104

An Eight Residue Fragment of an Acyl Carrier Protein Suffices for Post-Translational Introduction of Fluorescent Pantetheinyl Arms in Protein Modification in vitro and in vivo

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, and Department of Molecular and Cellular Biology and Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138

Water Is No Passive Spectator Of Biological Processes: It Is An Active Participant

Water is no passive spectator of biological processes; it is an active participant. Protein folding is thus a self-organized process in which the actions of the solvent play a key role. So far, the emphasis in studies of protein folding processes has been on observation of the protein backbone and its side chains.

Trump the clumps: Making biologic drugs safer

(Nanowerk News) Scientists at the National Institute of Standards and Technology (NIST) have developed a technique to measure the formation of clumps of proteins in protein-based pharmaceuticals. This first systematic study clarifies the conditions under which scientists can be assured that their instruments are faithfully measuring the formation of protein aggregates, a major concern because of its impact on quality control and safety in biologic drug manufacturing ("Determination of protein aggregation with differential mobility analysis: Application to IgG antibody").

Protein'Matrix Coupling/Uncoupling in €Dry€ Systems of Photosynthetic Reaction Center Embedded in Trehalose/Sucrose: The Origin of Trehalose Peculiarity

Protein−Matrix Coupling/Uncoupling in “Dry� Systems of Photosynthetic Reaction Center Embedded in Trehalose/Sucrose: The Origin of Trehalose Peculiarity

Protein's role in stem cells fate studied

Notch protein in human embryonic stem cells helps cells decide their own fate, which may be useful in stem cell therapy development, U.S. researchers said.

Protein Marker For Schizophrenia Risk

A protein found in immune cells may be a reliable marker for schizophrenia risk, report researchers in a new proteomics study.

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